Dr. Kay-Uwe Wagner was a member of our laboratory between 1995 and 2000. Kay received his Ph.D. in Animal Genetics at the University of Halle in Germany. In our lab Kay pioneered the Cre-loxP recombination system in mammary epithelium of the mouse and he was instrumental in the establishment of this system for several other cell types. Kay generated transgenic mice that express Cre recombinase under control of the mammary-specific WAP gene promoter and the MMTV-LTR. These mice are being now used my many investigators to inactivate genes, and have thus become an essential tool for the scientific community. Dr. Wagner also discovered that the cell survival protein Bcl-x is required for the establishment / maintenance of mature erythrocytes but that it is dispensable for immature erythrocytes (12). Dr. Wagner was supported by a grant from NIH and through funding from the German Research Society (DFG) and the DAAD.
In 2000 Dr. Wagner took a faculty position at the University of Nebraska Medical center in Omaha, NE. He studies, among other things, the role of TSG101 in the cell cycle. In 2004 Kay was promoted to Associate Professor. Wagner Lab.
Publications while in LGP
1. Cheung, A.M.Y., Elia, A., Tsao, M.-S., Done, S., Wagner, K.U., Hennighausen, L., Hakem, R. and Mak., T.W. (2004) Brca2 deficiency does not impair mammary epithelium development but promotes mammary adenocarcinoma formation in p53 +/- mutant mice. Cancer Research, in press.
2. Backman, S. A., Ghazarian, D., So, K., Sanchez, O., Wagner, K.U., Hennighausen, L., Khokha, R., Tsao, M.-S., Chapman, W.B., Stambolic, V. and Mak., T.W. (2004) Early onset of neoplasia in the prostate and skin of mice with tissue-specific deletion of PTEN. Proc. Natl. Acad. Sci. U.S.A., 101, 1725-1730.
3. Long, W., Wagner, K.U., Lloyd, K.C.K., Binart, N., Shillingford, J.M., Hennighausen, L. and Jones, F. (2003) Conditional deletion of Erbb4 in the mammary gland identifies ErbB4 as an obligate mediator of Stat5 activation and epithelial functional differentiation. Development, 130, 5257-5268.
4. Wagner, K.U., Krempler, A., Qi, Y., Park, K., Henry, M.D., Triplett, A.A., Riedlinger, G., Rucker, E.B. and Hennighausen, L. (2003) TSG101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissue, Mol. Cell. Biol., 23, 150-162.
5. Wagner, K.U., Boulanger, C.A., Henry, M.D., Sgagia, M., Hennighausen, L. and Smith, G.H. (2002) An adjunct mammary epithelial cell population in parous females: its role in functional adaptation and tissue renewal. Development, 129, 1377-1386.
6. Li, G., Robinson, G.W., Lesche, R., Martinez-Diaz, H., Jiang, Z., Rozengurt, N., Wagner, K.U., Wu, D.-C., Lane, T.F., Liu, X., Hennighausen, L. and Wu, H. (2002) Conditional deletion of Pten leads to precocious development and neoplasia in the mammary gland. Development, 129, 4158-4170.
7. Cui, Y., Miyoshi, K., Claudio, E., Siebenlist, U., Gonzalez, F., Flaws, J., Wagner, K.U. and Hennighausen, L. (2002) Loss of peroxisome proliferation-activated receptor gamma (PPARg) does not affect mammary gland development and propensity for tumor formation but leads to reduced fertility. J. Biol. Chem., 277, 17830-17835.
8. Riedlinger, G., Okagaki, R., Wagner, K.U., Rucker, E.B., Oka, T., Miyoshi, K., Flaws, J.A. and Hennighausen, L. (2002) Bcl-x is not required for the maintenance of follicles and the corpus luteum in the postnatal mouse ovary. Biology of Reproduction, 66, 438-444.
9. Miyoshi, K., Shillingford, J.M., Smith, G.H., Grimm, S.L., Wagner, K.U., Oka, T., Rosen, J.M., Robinson, G.W. and Hennighausen, L. (2001) Signal transducer and activator of transcription 5 (Stat5) controls the specification and proliferation of mammary alveolar epithelium. J. Cell Biol., 155, 531-542.
10. Robinson, G.W., Wagner, K.-U. and Hennighausen, L. (2001) Functional mammary gland development and oncogene-induced tumor formation are not affected by the absence of the retinoblastoma gene. Oncogene, 20, 7115-7119.
11. Yamashita, H., Nevalainen, M.T., X, J., LeBaron, M.J., Wagner, K.U., Erwin, R.A., Harmon, J.M., Hennighausen, L., Kirken, R.A. and Rui, H. (2001) Role of serine phosphorylation of Stat5a in prolactin-stimulated b-casein gene expression. Mol. Cell. Endocrinology 183, 151-163.
12. Walton, K.D., Wagner, K.-U., Rucker, E.B., Shillingford, J.M., Miyoshi, K. and Hennighausen, L. (2001) Conditional deletion of the bcl-x gene from mouse mammary epithelium results in accelerated apoptosis during involution but does not compromise cell function during lactation. Mech. of Development 109, 281-293.
13. Wagner, K.U., McAllister, K., Ward, T., Davis, B., Wiseman, R. and Hennighausen, L. (2001) Spatial and temporal expression of the Cre gene under the control of the MMTV-LTR in different lines of transgenic mice. Transgenic Research, 10, 545-553.
14. Wagner, K-U., Estefania C., Rucker, E., Riedlinger, G, Broussard, C., Schwartzberg, P.L., Siebenlist, U., Hennighausen, L. (2000) Conditional deletion of the bcl-x gene from erythroid cells results in hemolytic anemia and profound splenomegaly. Development, 127, 4949-4958.
15. Rucker, E., Dierisseau, P., Wagner, K.-U., Garrett, L., Wynshaw-Boris, A., Flaws, J. and Hennighausen, L., (2000). Bcl-x and Bax regulate mouse primordial germ cell survival and apoptosis during embryogenesis. Mol. Endo., 7, 1038-1052.
16. Xu, X., Wagner, K-U., Larson, D., Weaver, Z. Li, C., Ried, T. Hennighausen, L., Wynshaw-Boris, A. & Deng, C-X, (1999). Conditional mutation of Brca1 in mammary epithelial cells results in blunted ductal morphogenesis and tumor formation. Nature Genetics, 10, 37-43.
17. Wagner, K.-U., Dierisseau, P. and Hennighausen, L. (1999). Assigment of the murine tumor susceptibility gene 101 (tsg101) and a processed tsg101 pseudogene (tsg101-ps1) to mouse chromosome 7 band B5 and chromosome 15 band D1 by in situ hybridization. Cytogenet. Cell Genet. 84, 87-88.
18. Wagner, K.-U., Dierisseau, P., Rucker, III, E.B., Robinson, G.W. and Hennighausen, L. (1998) Genomic architecture and transcriptional activation of the mouse and human tumor susceptibility gene TSG101: Common types of shorter transcripts are true alternative splice variants. Oncogene, 17, 2761-2770.
19. Wagner, K.-U., Young III, W.S., Liu, X., Ginns, E., Li, M., Furth, P.A. and Hennighausen, L. (1997) Oxytocin and milk removal are required for post-partum mammary gland development. Genes and Function 1, 233-244. Newsletter of the UK Genetical Society
20. Wagner, K.-U., Wall, R.J., St-Onge, L., Gruss, P., Garrett, L., Wynshaw-Boris, A., Li, M., Furth, P.A. and Hennighausen, L. (1997) Cre mediated gene deletion in the mammary gland. Nucleic Acids. Res. 25, 4323-4330.
21. Li, M., Liu, X., Robinson, G.W., Bar-Peled, U., Wagner, K.-U., Young, W.S., Hennighausen, L. and Furth, P.A. (1997) Mammary derived signals activate programmed cell death in the involuting gland. Proc. Natl. Acad. Sci. U.S.A. 94, 3425-3430.
22. Liu, X., Robinson, G.W., Wagner, K.-U., Garrett, L., Wynshaw-Boris, A. and Hennighausen, L. (1997) Stat5a is mandatory for adult mammary gland development and lactogenesis. Genes and Dev. 11, 179-186.
23. Young, S.W., Shepard, E., Amico, J., Hennighausen. L., Wagner, K.-U., LaMarca, M.E., McKinney, C. and Ginns, E.I. (1996) Deficiency in mouse oxytocin prevents milk ejection, but not fertility or parturition. J. Neuroendocrinology 8, 847-853.
24. Wagner, Rucker, E.B. and Hennighausen, L. (2000) Adenoviral and transgenic approaches for the conditional deletion of genes from mammary tissue. Methods in Mammary Gland Biology and Breast Cancer Research, Kluwer Academic / Plenum Publishers, New York, pages 271-284.